Biochemistry

Group
Il Gruppo di ricerca si prefigge di isolare, identificare e caratterizzare da un punto di vista biochimico le macromolecole e i flavonoidi e studiarne le reciproche interazione e possibili applicazioni biologiche
date/time interval:
(November 1, 2020 - )

Overview

Acronym

BICME

Term type

Gruppo di ricerca coordinata

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Concepts (13)

Overview (9)

Bbiochemistry and physiology adaptation of arginine pathway in marine organism (with particular regard to elasmobranches)
Biomolecules stabilization processes by sugar, salts, ions and biopolimers through chromatographic, spectroscopic and electrophoresis techniques, analyzing chemical-physic variations of solvent and macromolecular solution
Dynamic and structural correlations between proteins and solvent, in the absence or in the presence of kosmotropes and chaotropes, following folding or unfolding, dehydration and preservation, with the purpose to clarify the relationship functionality/structure
Heavy metal pollution analyzing biochemistry and physiology modifications due to growh in their presence ─ valuation of main pathway involved in celss after
Isolation and identification of some poliphenols of natural source and antioxidant and pro-oxidant activity determination by cell culture (HEP, HEP G2, VERO, erythrocytes and lymphocytes) assay
Protein’s thermodynamic and kinematic parameters (enthalpy, entropy, DG of folding/unfolding, Wyman equation) determination in absence or presence of physical or chemical stresses
Purification and characterization of protein in particular on ornithine carbamoyltransferase, isolated from different organisms and folding/unfolding studies of the same enzyme
Studies on erythrocyte membrane, hemolysis assay, lipid peroxidation, membrane fluidity, Band-3 functionality, evaluation of metabolic energy fluxes
Valuation of main pathway involved in celss after biological and chemical hazard by enzyme isomorphism profile (lactic and malic dehydrogenase, seperoxide dismutase, caspase, glutamic oxalacetic transaminase) membrane properties and functions, primary and secondary metabolites
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