Dietary mixed and sprayed probiotic improves growth performance and digestive enzymes of juvenile whiteleg shrimp (Litopenaeus vannamei, Boone, 1931)

This study investigated the effects of dietary mixed and sprayed of Bacillus subtilis subsp. inaquosorum IS02 (B.s.i. IS02) on growth performance and digestive enzyme activity of whiteleg shrimp. Three different dosages of B.s.i. IS02 were applied via two application methods: mixed with (TM) and sprayed on (TS) feed containing 10(8) (TM1 and TS1), 10(7) (TM2 and TS2), 10(6) (TM3 and TS3) CFU Kg(-1). Compared to control groups, growth performance was significantly higher in treated shrimp. There was a significant difference in survival rate between TM1 (94.44 +/- 1.57%) and TM2 (87.78 +/- 1.92%) compared to the other diets. Furthermore, the activity of digestive enzymes was significantly higher in TS1. Overall, it can be concluded that spraying B.s.i. IS02 at the dosage of 10(7) or 10(8) CFU kg(-1) on feed can be a useful method in the farm; also, mixing at the dose of 10(8) CFU kg(-1) may prove effective in feed factories.